Charlyne was born in Los Angeles and began performing in Fontana, California. She has performed many shows in the Los Angeles area in venues including the Steve Allen Theater and the Upright Brigade Theater. In 2005 and 2006, Charlyne performed in the New York Comedy Festival and in HBO's 2007 US Comedy Arts Festival in Aspen, Colorado. Yi made her film debut in the 2007 film Knocked Up, starring Katherine Heigl and Seth Rogan. She stars in the 2009 documentary Paper Heart, where she plays a fictionalized version of herself alongside Michael Cera.
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